Maxwell® CSC XtractAll FFPE DNA/RNA Kit
Efficient and Reliable Automated Extraction of DNA and RNA from FFPE Tissues for Amplification-Based Diagnostic Applications
- Extract both DNA and RNA from the same formalin-fixed, paraffin-embedded (FFPE) tissue sample—no need to split the sample
- Extract DNA, RNA, Total Nucleic Acid or both DNA and RNA sequentially in separate tubes—one versatile kit with four workflows
- Maximize genomic and transcriptomic information while conserving FFPE tissue samples
- Achieve fast and streamlined workflow—go from FFPE tissue sections to purified DNA and RNA in 3 hours or less
- cGMP-manufactured and compliant with relevant IVD medical device regulations, including Regulation (EU) 2017/746 on In Vitro Diagnostic Medical Devices (IVDR) and US IVD regulations
Catalog Number:
Size
Turn FFPE Tissues into Actionable Insights with High-Yield, High-Purity DNA and RNA Extraction in a Seamless Automated Workflow
The Maxwell® CSC XtractAll FFPE DNA/RNA Kit transforms FFPE tissue sample extraction with unmatched flexibility and speed. Designed for use with Maxwell® CSC Instruments, this all-in-one kit delivers high-quality DNA, RNA or both while reducing FFPE tissue consumption in an automated workflow. With optimized chemistry and workflow-specific preprogrammed Maxwell® CSC XtractAll FFPE methods, the kit supports four complete workflows for nucleic acid extraction from FFPE tissue samples: 1) DNA, 2) RNA, 3) Total Nucleic Acid or 4) DNA and RNA sequential extraction in separate eluates from the same FFPE tissue section.
Process 1–16 or 1–48 samples simultaneously on the Maxwell® CSC Instrument or the Maxwell® CSC 48 Instrument, respectively, boosting lab efficiency and time-savings. The Maxwell® CSC XtractAll FFPE DNA/RNA Kit is a versatile turnkey solution offering you the flexibility to tailor nucleic acid extraction from FFPE samples to fit your clinical workflow needs.
Prefilled Maxwell® CSC XtractAll FFPE cartridges contain all the necessary reagents required for effective purification of nucleic acids by eliminating contaminants and inhibitors, delivering consistent results in every extraction. DNA, RNA or total nucleic acid are extracted and purified with uncompromised integrity, reducing assay failures and enhancing confidence in clinical assay results. The Maxwell® CSC XtractAll FFPE DNA/RNA Kit is validated for in vitro diagnostic use and delivers high-quality nucleic acids ready for use in a wide range of sensitive downstream applications such as real-time PCR, digital PCR and next-generation sequencing (NGS).
Available in Austria, Belgium, Canada, Croatia, Czech Republic, Denmark, Finland, France, Germany, Greece, Hungary, Ireland, Italy, Latvia, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Singapore, Slovakia, Slovenia, Spain, Sweden, Switzerland, United Kingdom and United States.
A Versatile Solution for All your FFPE Tissue Nucleic Acid Extraction Needs—Tailor your Clinical Workflow with Maximum Flexibility
The Maxwell® CSC XtractAll FFPE DNA/RNA Kit enables four distinct nucleic acid extraction workflows from FFPE tissues using a single, streamlined preprocessing protocol with just a few simple steps.
- Prepare FFPE Tissue Lysate: A simple pretreatment protocol streamlines FFPE tissue deparaffinization, lysis and nucleic acid decrosslinking in under 2 hours, reducing hands-on time and effort.
- Prepare Maxwell® Cartridge: Add FFPE tissue lysate and workflow-specific reagents as needed (refer to Technical Manual #TM755) and insert a plunger into Maxwell® CSC cartridge. Only one cartridge and plunger are needed per sample, regardless of the workflow, reducing plastic waste.
- Start automated extraction method: Load the prepared cartridges, with elution tubes containing 50µl of Nuclease-Free Water, in the Maxwell® CSC or Maxwell® CSC 48 Instrument, and start the workflow-specific preprogrammed Maxwell® CSC XtractAll FFPE method with the touch of a button.
Note: Before using the Maxwell® CSC XtractAll FFPE DNA/RNA Kit, ensure that:
- Software version 4.0.0 or higher and Maxwell® CSC XtractAll FFPE methods are installed on the Maxwell® CSC Instrument (download here).
- Software version 4.1.1 or higher and Maxwell® CSC XtractAll FFPE methods are installed on the Maxwell® CSC 48 Instrument (download here).
- Enjoy walkaway convenience: Maxwell® CSC Instruments perform all nucleic acid extraction and purification steps within the prefilled reagent cartridge, yielding concentrated, high-quality nucleic acids. Singular DNA, RNA or total nucleic acid are purified in 30 minutes or less; DNA and RNA sequential extraction is complete in under 1 hour.
Simplified DNA and RNA extraction from FFPE tissues. The Maxwell® CSC XtractAll FFPE DNA/RNA Kit enables efficient extraction of DNA and RNA from diverse FFPE tissues—saving time, effort and precious sample material. For detailed information and a protocol for Maxwell® CSC XtractAll FFPE DNA/RNA Kit workflows, refer to Technical Manual #TM755.
Streamline FFPE Tissue Extraction— Process More Samples in Less Time with No Compromises
The Maxwell® CSC XtractAll FFPE DNA/RNA Kit delivers fast, high-yield automated extraction of both DNA and RNA sequentially from FFPE tissue samples, completed in under 3 hours. The streamlined protocol simplifies FFPE sample extraction workflow by minimizing hands-on time and reducing complexity, empowering clinical labs to process more samples with greater efficiency. Turnaround time for critical results is accelerated without compromising quality—ideal for fast-paced diagnostic and clinical research environments.
Maximize time and efficiency. The Maxwell® CSC XtractAll FFPE DNA/RNA Kit paired with the Maxwell® CSC Instruments minimizes hands-on time, reduces errors and enables accelerated turnaround as compared to other manual FFPE DNA/RNA extraction kits.
The Maxwell® CSC XtractAll FFPE DNA/RNA Kit offers flexible nucleic acid extraction with four workflow options, each delivering high performance without compromising yield or quality. This versatility and robust performance streamlines sample processing for clinical applications that require both genomic and transcriptomic data, enables laboratories to tailor nucleic acid purification for a variety of downstream applications, reduces consumption of precious FFPE tissue samples and minimizes inventory management.
Efficient recovery of high-yield nucleic acid from FFPE tissues across all workflows. DNA, RNA or Total Nucleic Acid were extracted from FFPE human lung tumor (Panel A) and FFPE human colon (Panel B) tissue samples using the four different workflows of the Maxwell® CSC XtractAll FFPE DNA/RNA Kit: 1) DNA, 2) RNA, 3) DNA/RNA sequential (‘Seq DNA’ and ‘Seq RNA’) and 4) total nucleic acid (TNA). DNA yield was determined using qPCR for amplification of a 102bp target, while RNA yield was evaluated by RT-qPCR amplifying a 100bp amplicon. Data shown as mean ± SD, N = 6. The TNA and DNA/RNA sequential workflows achieved comparable yields to singular DNA and RNA extractions, offering flexibility without compromising recovery.
Maximize Confidence in Sensitive Molecular Applications with High-Quality DNA/RNA from Challenging FFPE Tissue Samples
The Maxwell® CSC XtractAll FFPE DNA/RNA Kit maximizes recovery of high-quality amplifiable DNA and RNA with high integrity from a variety of FFPE tissue types, including challenging samples. DNA and RNA are suitable for demanding downstream molecular applications such as NGS, digital PCR and more, supporting greater sensitivity and confidence in diagnostic and clinical research workflows.
High-yield and high-quality DNA and RNA extraction across diverse FFPE tissue types using the Maxwell® CSC XtractAll FFPE DNA/RNA Kit. DNA and RNA were extracted from a single section of each indicated FFPE human tissue type using either the Maxwell® CSC XtractAll FFPE DNA/RNA sequential workflow or two alternative manual kits following manufacturer protocols. DNA yield was assessed by qPCR targeting 150bp (Panel A) and 300bp (Panel B) amplicons. RNA yield was measured by RT-qPCR targeting 100bp (Panel C) and 171bp (Panel D) amplicons. Across all FFPE tissue types, the Maxwell® CSC XtractAll FFPE DNA/RNA Kit delivered comparable or higher DNA and RNA yields, particularly for longer amplicons, demonstrating its robust performance and effectiveness in recovering high-quality, amplifiable nucleic acids from challenging FFPE tissue samples. Data represent mean ± SD, N = 6.
Superior DNA and RNA fragment quality from FFPE tissue using the Maxwell® CSC XtractAll FFPE DNA/RNA Kit. DNA and RNA were extracted from single sections of a FFPE human breast tissue using the Maxwell® CSC XtractAll FFPE DNA/RNA sequential workflow and two other alternative FFPE DNA/RNA kits. Fragment quality was assessed using TapeStation analysis. Panel A. DNA fragment quality was assessed using the Agilent D5000 ScreenTape Assay. Panel B. RNA fragment quality and DV200 values were assessed using the Agilent RNA ScreenTape Assay. These results demonstrate that the Maxwell® CSC XtractAll FFPE DNA/RNA Kit yields high-quality, intact DNA and RNA with greater integrity compared to competitor kits, which show lower integrity and increased DNA and RNA fragmentation.
The high quality and amplifiability of DNA and RNA extracted from FFPE tissue samples obtained using the Maxwell® CSC XtractAll FFPE DNA/RNA Kit enables sensitive and reliable gene expression or mutational analysis in clinical and translational workflows.
Detection of oncogenic variants by digital PCR using RNA extracted from FFPE tissue. RNA was extracted from different FFPE human lung tumor tissue samples using the Maxwell® CSC XtractAll FFPE DNA/RNA sequential workflow and analyzed using a droplet digital PCR assay for EML4-ALK mRNA fusions. A representative 2D amplitude scatter plot displays the distinct cluster of droplets in the upper-left quadrant (in blue circle labeled as ‘Positive’), indicating successful amplification of the target gene fusion variant. Negative droplets are concentrated near the baseline, confirming strong signal separation and assay specificity.
| DNA Sequencing | |||||||
|---|---|---|---|---|---|---|---|
| FFPE Human Tumor Tissue | Extraction Kit | QC Metrics | Sequencing Metrics | ||||
| DIN | Total PF Reads | Aligned Reads (%) | Median Target Coverage | Median Insert Size | Usable MSI Sites | ||
| Breast | XtractAll | 5.8 | 127,995,636 | 98.4 | 805 | 143 | 126 |
| Competitor Q | 3.7 | 136,736,976 | 99.5 | 931 | 126 | 126 | |
| Lung | XtractAll | 5.7 | 145,314,910 | 98.3 | 1055 | 131 | 126 |
| Competitor Q | 2.6 | 139,588,616 | 96.4 | 307 | 97 | 124 | |
| RNA Sequencing | |||||||
|---|---|---|---|---|---|---|---|
| FFPE Human Tumor Tissue | Extraction Kit | QC Metrics | Sequencing Metrics | ||||
| RIN | DV200 (%) | Total PF Reads | Gene Median Coverage | Median Insert Size | Splice Variants | ||
| Breast | XtractAll | 2.6 | 84.36 | 127,138,838 | 1269 | 133 | 2 |
| Competitor Q | 2.5 | 60.08 | 112,467,372 | 476 | 106 | 0 | |
| Lung | XtractAll | 2.1 | 77.45 | 128,484,884 | 2047 | 115 | 5 |
| Competitor Q | 2.2 | 44.18 | 141,567,206 | 324 | 90 | 0 | |
DNA and RNA compatible with next-generation sequencing (NGS). DNA and RNA were extracted from a single section of FFPE human breast- and lung-tumor tissues using either the Maxwell® CSC XtractAll FFPE DNA/RNA sequential workflow (XtractAll) or a competitor FFPE DNA/RNA extraction kit (Competitor Q). Purified DNA and RNA samples were analyzed using the TruSight Oncology 500 (TSO 500) Assay (Illumina). Successful library preparation was achieved for all the samples, and sequencing was performed on the NovaSeq PE150 platform (Illumina), generating 80 million total reads per sample (40 million paired-end reads) at a coverage depth of 35,000X. Key quality control and NGS performance metrics for DNA and RNA extracted from the indicated FFPE tissue samples using the Maxwell® CSC XtractAll FFPE DNA/RNA Kit versus Competitor Q are presented. Compared to Competitor Q, the Maxwell® CSC XtractAll FFPE DNA/RNA Kit produced DNA and RNA with higher integrity and fragment length that exhibited better target coverage.
Reliable Fluorometric Quantitation that Translates to Downstream Assay Success
Fluorometric assays using DNA- and RNA-specific dyes are routinely used in clinical laboratories to estimate nucleic acid yield. Unlike other FFPE extraction methods, the Maxwell® CSC XtractAll FFPE DNA/RNA Kit delivers high-quality DNA and RNA whose fluorometric quantitation closely correlates with actual assay performance in downstream applications such as qPCR and digital PCR. This strong concordance between fluorometric yield estimation and amplification assay success supports improved workflow predictability, minimizes the risk of inconclusive or failed results—saving your lab valuable time, resources and repeat testing, and boosting confidence in molecular testing from challenging FFPE tissue samples.
Improved concordance between quantitation and amplification performance. DNA and RNA were extracted from eight distinct FFPE tissue samples using the Maxwell® CSC XtractAll FFPE DNA/RNA sequential workflow and two alternative manual FFPE DNA/RNA extraction kits, following each manufacturer’s protocol. Panel A. DNA yield was assessed via fluorometric quantitation using a double-stranded DNA-specific dye and by qPCR amplification targeting a 164bp fragment. Panel B. RNA yield was quantified using a fluorometric assay with an RNA-selective dye and by RT-qPCR targeting a 100bp amplicon. Box plots represent the ratio of fluorometric to amplification-based quantitation (Fluorometric/Amp) for both DNA and RNA. Ratio values closer to 1 reflect stronger concordance between yield estimation and actual assay performance. Compared to alternative kits, the Maxwell® CSC XtractAll FFPE DNA/RNA Kit consistently demonstrated lower and narrower ratio distributions, indicating more reliable fluorometric quantitation and greater consistency in downstream amplification-based molecular assays.
Protocols
Specifications
Catalog Number:
What's in the box?
| Item | Part # | Size | Available Separately |
|---|---|---|---|
CSC/RSC Plungers |
AS133A | 1 × 50/pk | View Product |
Maxwell® CSC Cartridge (CSCR) |
AS156B | 48 × 1 each | |
Elution Tubes (0.5ml) |
AS620A | 2 × 50/pk | View Product |
Proteinase K (PK) Solution |
MC111A | 2 × 1ml | |
Lysis Buffer |
MC118A | 1 × 20ml | |
Nuclease-Free Water |
MC119A | 1 × 25ml | |
Mineral Oil |
MC121B | 1 × 35ml | |
Blue Dye |
MC122A | 2 × 100μl | |
MnCl2, 0.09M |
MC125A | 2 × 1ml | |
DNase I (lyophilized) |
Z358A-C | 3 × 1 vial |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For In Vitro Diagnostic Use. This product is only available in certain countries.Storage Conditions
Related Products
Similar Products
Maxwell® CSC Rapid ccfDNA Kit
Automated ccfDNA extraction in under 30 minutes with high yield, low contamination, and fast turnaround.
AS1580
Maxwell® CSC DNA FFPE Kit
Isolates DNA from FFPE samples for in vitro diagnostic assays using the Maxwell® CSC Instrument.
AS1350
Maxwell® CSC RNA FFPE Kit
Purifies RNA from FFPE tissue samples for downstream in vitro diagnostic assays using the Maxwell® CSC Instrument.
AS1360
Frequently Used With
Maxwell® CSC Instrument
Benchtop instrument for automated nucleic acid purification from a range of clinical sample types. For In Vitro Diagnostic Use.
AS6000
Maxwell® CSC 48 Instrument
Benchtop instrument for automated nucleic acid purification from a range of clinical sample types. For In Vitro Diagnostic Use.
AS8000
