Maxwell® HT Rapid ccfDNA Kit
Fast, Scalable and Automation-Ready High-Throughput ccfDNA Extraction
- High Throughput Workflow: Purify up to 96 samples per run with minimal pipetting and hands-on time
- Flexible Volumes: Process 1–4ml of input plasma
- Compatible with KingFisher™ Apex and automated liquid handlers
- Consistent Results: High ccfDNA yields with low gDNA contamination—ideal for precision assays
- Scalable Chemistry: Same purification chemistry as the Maxwell® RSC Rapid ccfDNA Kit for flexible throughput
Catalog Number:
Size
Overview
The Maxwell® HT Rapid ccfDNA Kit delivers robust and efficient purification of circulating cell-free DNA (ccfDNA) from up to 4ml of plasma per sample, enabling high-throughput workflows on automated platforms. Designed for research labs processing large sample sets, this kit provides high yields of low-gDNA ccfDNA ideal for sensitive downstream applications including digital PCR, NGS and qPCR.
Streamlined Workflow for Lab Automation
The Maxwell® HT Rapid ccfDNA Kit includes bulk reagents and a simplified, no-pretreatment protocol for straightforward automation. From load to elution, the system enables researchers to move from raw plasma to high-quality ccfDNA with rapid protocols. Pending instrument configuration, liquid handlers can process up to 96 four-milliliter (4ml) samples in less than 2 hours, and Kingfisher™ instruments can process 24 four-milliliter (4ml) samples in about 30–35 minutes.
Expert Automation Support—Included at No Additional Cost
When you choose Promega reagents, you gain more than just reliable chemistry. Our dedicated team of automation experts provides complimentary support to streamline your implementation. This includes assistance with liquid handler programming, instrument method installation and hands-on training for your laboratory staff—ensuring a seamless transition to high-throughput ccfDNA extraction on your chosen platform.
Data-Backed Performance
- Yield scales linearly from 1–4ml inputs.
- Consistent fragment size profiles for high-quality ccfDNA suitable for NGS.
- Validated on common platforms for robust and reproducible results.
For Lower Throughput Workflows
For researchers processing fewer samples, consider the Maxwell® RSC Rapid ccfDNA Kit (Cat.# AS1590)—optimized for 1–16 samples on the Maxwell® RSC Instrument and 1–48 samples on the Maxwell® RSC 48 Instrument.
Protocols
No protocols available
Specifications
Catalog Number:
What's in the box?
Item | Part # | Size |
---|---|---|
Proteinase K (PK) Solution |
MC111A | 1 × 1ml |
Binding Buffer (RCFD) |
MC155 | 1 × 76ml |
Maxwell® Resin (RCFD) |
MC156 | 1 × 45ml |
Wash Buffer (RCFD1) |
MC157 | 1 × 105ml |
Wash Buffer (RCFD2) |
MC158 | 1 × 110ml |
Elution Buffer (RCFD) |
MC159 | 1 × 40ml |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
Resources
Other Resources
- Nucleic Acid Purification Application Notes Portal
- Lab Automation Resource Center
- Webinar: Automation Experts Answer Your FFPE, Liquid/Tissue Biopsy DNA and RNA Extraction Questions Live
- Webinar: Maxwell® Rapid ccfDNA Kit: Enhanced Efficiency and Quality in Circulating Cell-Free DNA Extraction
- Webinar: Ask the Experts: How to Automate Nucleic Acid Extraction
- Webinar: To NanoDrop® or Not to NanoDrop®: Choosing the Most Appropriate Method for Nucleic Acid Quantitation
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Check the gDNA extracted from potentially degraded sources or ccfDNA samples for both quality and quantity.
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