ProNex® DNA QC Assay BioRad CFX96™
Evaluate Quality and Quantity of gDNA from Degraded Samples
- Human-specific, multiplexed probe-based qPCR assay with internal positive control
- Detect 75bp, 150bp and 300bp human genomic DNA sequences
- Evaluate your samples for amplifiability and predict downstream assay success
Catalog Number:
Size
Catalog Number: NG1004
Catalog Number: NG1005
Qualify and Quantify Your Samples in a Single Reaction
The ProNex® DNA QC Assay evaluates the quality and quantity of genomic DNA extracted from formalin-fixed paraffin-embedded (FFPE) samples or other potentially degraded DNA sources. It is a human-specific, multiplexed probe-based quantitative polymerase chain reaction (qPCR) assay that may also be used to evaluate the ratio of circulating cell-free DNA (ccfDNA) to higher molecular weight genomic DNA in plasma samples. The multiplex assay detects 75bp, 150bp and 300bp human genomic DNA sequences, and it includes an internal positive control (IPC) to test for false-negative results that may occur in the presence of PCR inhibitors.
One Step Toward a Better NGS Workflow
Comparison of Quantitation and Quality Control Features
Not all QC analysis is the same. Only the ProNex® DNA QC Assay includes everything you need, with minimal sample usage.
Downstream Predictive Value for Degraded Samples
Due to the inherent variability of FFPE DNA quality, knowing the quantity of DNA alone is not reliably predictive of downstream assay success. The ProNex® DNA QC Assay allows you to determine the amount of amplifiable DNA in a sample. You can use the ProNex® DNA QC Assay to evaluate if a sample is suitable for downstream analysis using techniques such as next-generation sequencing (NGS) or droplet digital PCR (ddPCR).
Evaluate ccfDNA Samples for gDNA Contamination
Process Your Data with the ProNex® DNA QC Assay Analysis Software
The ProNex® DNA QC Assay Analysis Software will process qPCR data generated with the ProNex® DNA QC Assay Kits. The software includes calculations and methods to define values for the following:
- Standard Curves (acceptable r2 and slope values)
- Sample quantity (based on the Standard Curves)
- Sample quality (possible inhibition, contamination, or degradation)
The ProNex® DNA QC Assay Analysis Software can import results from the Applied Biosystems® 7500, 7500 Fast or QuantStudio™ 6 Flex Real-Time PCR Systems, and the BioRad CFX96 Touch™ Real-Time PCR Detection System.
Specifications
Catalog Number:
What's in the box?
| Item | Part # | Size | Concentration | Available Separately |
|---|---|---|---|---|
ProNex® DNA QC Dilution Buffer |
NG101A | 2 × 1.5ml | ||
2X ProNex® DNA QC Master Mix |
NG121A | 2 × 1.1ml | ||
20X ProNex® DNA QC Primer-Probe-IPC Mix, BioRad CFX96™ |
NG151A | 1 × 220μl | ||
ProNex® DNA QC gDNA Standard |
NG302A | 1 × 150μl | 50ng/μl | |
|
Nuclease-Free Water |
P119A | 2 × 1,250μl | View Product |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
Dye compounds in this product are sold under license from Biosearch Technologies, Inc., and protected by U.S. and worldwide patents either issued or in application. The license does not include rights for human IVD use.
What's in the box?
| Item | Part # | Size | Concentration | Available Separately |
|---|---|---|---|---|
ProNex® DNA QC Dilution Buffer |
NG101A | 8 × 1.5ml | ||
2X ProNex® DNA QC Master Mix |
NG121A | 8 × 1.1ml | ||
20X ProNex® DNA QC Primer-Probe-IPC Mix, BioRad CFX96™ |
NG151A | 4 × 220μl | ||
ProNex® DNA QC gDNA Standard |
NG302A | 4 × 150μl | 50ng/μl | |
|
Nuclease-Free Water |
P119A | 8 × 1,250μl | View Product |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
Dye compounds in this product are sold under license from Biosearch Technologies, Inc., and protected by U.S. and worldwide patents either issued or in application. The license does not include rights for human IVD use.
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Frequently Used With
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Fast, manual method for purifying DNA from FFPE tissue without using organic solvents.
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